ASCLD - Validation & Evaluation Repository

The ASCLD Forensic Research Committee is proud to announce the launch of the Validation and Evaluation Repository. The goal is to compile a list of unique validations and evaluations conducted by forensic labs and universities. The repository will catalog these efforts and provide the contact information of the people responsible for the work. It is ASCLD’s hope that this listing will foster communication and reduce unnecessary repetition of validations and evaluations to benefit the forensic community. If you find a validation or evaluation that you would like more information about, simply use the contact information provided to request a copy.

If you would like to submit information for the Repository, please click HERE.

Please use the following items to search the entries. You can use as many of the following as you need:

Validation Report for Quantitation and/or Qualitative Identification of Benzodiazepines, Opioids and Select Hypnotics in Whole Blood by LC MSMS

Keywords: Opioids, Benzodiazepines, Hypnotics, LC-MSMS, Whole Blood
Laboratory: Palm Beach County Sheriff’s Office Crime Laboratory
Discipline: Toxicology
Contact Name: Nick Tiscione
Email: tiscionen@nullpbso.org
File link: https://www.ascld.org/wp-content/uploads/formidable/13/Validation-Report-for-Quantitation-and_or-Qualitative-Identification-of-Benzodiazepines-Opioids-.pdf
Web link:

Validation Report for Quantitation of Select Stimulants in Whole Blood by LC-MSMS

Keywords: Stimulants, LC-MSMS, Whole Blood
Laboratory: Palm Beach County Sheriff’s Office Crime Laboratory
Discipline: Toxicology
Contact Name: Nick Tiscione
Email: tiscionen@nullpbso.org
File link: https://www.ascld.org/wp-content/uploads/formidable/13/Validation-Report-for-Quantitation-of-Select-Stimulants-in-Whole-Blood-by-LC-MSMS.pdf
Web link:

Validation Summary for Alcohol Analysis by Headspace GC-FID/MS

Keywords: BAC, ethanol, Headspace
Laboratory: Palm Beach County Sheriff’s Office Crime Laboratory
Discipline: Toxicology
Contact Name: Nick Tiscione
Email: tiscionen@nullpbso.org
File link: https://www.ascld.org/wp-content/uploads/formidable/13/Validation-Summary-for-Alcohol-Analysis-by-Headspace-GC-FIDMS.pdf
Web link:

ForenSeq Kintelligence Kit, MiSeq FGx Sequencing, and Universal Analysis Software Internal Validation

Keywords: Kintelligence, Single nucleotide polymorphism (SNP), Massively Parallel Sequencing (MPS), Forensic genetic genealogy (FGG)
Laboratory: Signature Science, LLC – Center for Advanced Genomics
Discipline: Biology/Serology
Contact Name: Michelle Peck
Email: mpeck@nullsignaturescience.com
File link:
Web link: https://www.liebertpub.com/doi/10.1089/forensic.2022.0014

NYC OCME Internal Validation of STRmixTM v2.7 for Fusion 5C/3500xL Data – STRmixTM Parameters

Keywords: STRmix, Fusion 5C/3500XL
Laboratory: New York City Office of the Chief Medical Examiner
Discipline: Biology/Serology
Contact Name: Timothy D. Kupferschmid
Email: tkupferschmid@nullocme.nyc.gov
File link:
Web link: https://www1.nyc.gov/assets/ocme/downloads/pdf/strmix_v2_7_parameters.pdf

Estimation of STRmix parameters for OCME New York Laboratory

Keywords: STRmix
Laboratory: New York City Office of the Chief Medical Examiner
Discipline: Biology/Serology
Contact Name: Timothy D. Kupferschmid
Email: tkupferschmid@nullocme.nyc.gov
File link:
Web link: https://www1.nyc.gov/assets/ocme/downloads/pdf/strmix_parameters.pdf

Internal Validation of STRmix V2.4 for Fusion

Keywords: STRmix
Laboratory: New York City Office of the Chief Medical Examiner
Discipline: Biology/Serology
Contact Name: Timothy D. Kupferschmid
Email: tkupferschmid@nullocme.nyc.gov
File link:
Web link: https://www1.nyc.gov/assets/ocme/downloads/pdf/STRmix-V2-4-Fusion-5C-Validation%20Summary.pdf

Internal Validation of GeneMarker HID software

Keywords: GeneMarker, HID
Laboratory: New York City Office of the Chief Medical Examiner
Discipline: Biology/Serology
Contact Name: Timothy D. Kupferschmid
Email: tkupferschmid@nullocme.nyc.gov
File link:
Web link: https://www1.nyc.gov/assets/ocme/downloads/pdf/GeneMarker-HID-Validation-Summary.pdf

PowerPlex® Fusion System Amplification Kit on the Applied Biosystems 3130xl Genetic Analyzer using GeneMarker HID v. 2.8.2 Analysis Software Validation Report

Keywords: PowerPlex Fusion, 3130xL
Laboratory: New York City Office of the Chief Medical Examiner
Discipline: Biology/Serology
Contact Name: Timothy D. Kupferschmid
Email: tkupferschmid@nullocme.nyc.gov
File link:
Web link: https://www1.nyc.gov/assets/ocme/downloads/pdf/Fusion-Validation-Summary.pdf

Internal Validation of STRmix™ v2.7 for Fusion 5C/3500xL Data

Keywords: STRmix, Fusion 5C/3500xL
Laboratory: New York City Office of the Chief Medical Examiner
Discipline: Biology/Serology
Contact Name: Timothy D. Kupferschmid
Email: tkupferschmid@nullocme.nyc.gov
File link:
Web link: https://www1.nyc.gov/assets/ocme/downloads/pdf/internal_validation_strmix_2_7.pdf

Multi-drug Screen in Blood and Urine by LC/MS/MS Using SLE+ Extraction Plates

Keywords: Toxicology, Multi-Drug, LC/MS/MS
Laboratory: Idaho State Police Forensic Services
Discipline: Toxicology
Contact Name: Matthew Gamette
Email: matthew.gamette@nullisp.idaho.gov
File link: https://www.ascld.org/wp-content/uploads/formidable/13/Validation-Summary-MDS-APPROVED.pdf
Web link: https://isp.idaho.gov/forensics/validation/

Multi-drug and Cannabinoids Screen in Blood and Urine by LC/Q-TOF Using SLE+ Extraction Plates

Keywords: Toxicology, LC/Q-TOF, Multi-Drug, Cannabinoids
Laboratory: Idaho State Police Forensic Services
Discipline: Toxicology
Contact Name: Matthew Gamette
Email: matthew.gamette@nullisp.idaho.gov
File link: https://www.ascld.org/wp-content/uploads/formidable/13/Final-Validation-Summary-QTOF-MDS-APPROVED.pdf, https://www.ascld.org/wp-content/uploads/formidable/13/Final-Validation-Summary-QTOF-Cannabinoids-APPROVED.pdf
Web link: https://isp.idaho.gov/forensics/validation/

Antiepileptics / Acidic Drug Confirmation and Quantitation by LCMSMS

Keywords: antiepileptics, acidic drugs, LCMSMS
Laboratory: Montgomery County Coroner’s Office / Miami Valley Regional Crime Laboratory
Discipline: Toxicology
Contact Name: Matthew Juhascik
Email: juhascikm@nullmcohio.org
File link: https://www.ascld.org/wp-content/uploads/formidable/13/AEA-by-LCMSMS-Summary-of-Validation-7482-1-1.pdf
Web link:

Hemp analysis by LCMSMS

Keywords: hemp, marijuana, total THC, LCMSMS
Laboratory: Montgomery County Coroner’s Office / Miami Valley Regional Crime Laboratory
Discipline: Seized Drugs
Contact Name: Matthew Juhascik
Email: juhascikm@nullmcohio.org
File link: https://www.ascld.org/wp-content/uploads/formidable/13/Hemp-THC-and-THCA-by-LCMSMS-Summary-of-Validation-7489-1.pdf
Web link:

Drug-Facilitated Crime Analysis by LCMSMS

Keywords: drug-facilitated, sexual assault, LCMSMS, solid-phase extraction
Laboratory: Montgomery County Coroner’s Office / Miami Valley Regional Crime Laboratory
Discipline: Toxicology
Contact Name: Matthew Juhascik
Email: juhascikm@nullmcohio.org
File link: https://www.ascld.org/wp-content/uploads/formidable/13/DFC-Validation-Summary-7487-1.pdf
Web link:

THC related compounds by LCMSMS

Keywords: THC, delta 8, LCMSMS, solid-phase extraction
Laboratory: Montgomery County Coroner’s Office / Miami Valley Regional Crime Laboratory
Discipline: Toxicology
Contact Name: Matthew Juhascik
Email: juhascikm@nullmcohio.org
File link: https://www.ascld.org/wp-content/uploads/formidable/13/THCPlus-by-LCMSMS-Summary-of-Validation-7494-1.pdf
Web link:

Benzodiazepines by LCMSMS

Keywords: Benzodiazepines, LCMSMS, solid-phase extraction
Laboratory: Montgomery County Coroner’s Office / Miami Valley Regional Crime Laboratory
Discipline: Toxicology
Contact Name: Matthew Juhascik
Email: juhascikm@nullmcohio.org
File link: https://www.ascld.org/wp-content/uploads/formidable/13/Benzo-by-LCMSMS-Summary-of-Validation-7486-1.pdf
Web link:

ProFlex Thermalcycler Validation with GlobalFiler

Keywords: ProFlex, PCR, Thermalcycler, Globalfiler, Yfiler
Laboratory: Colorado Bureau of Investigation
Discipline: Biology/Serology
Contact Name: Jennifer Malone, DNA Technical Leader
Email: jennifer.malone@nullstate.co.us
File link: https://www.ascld.org/wp-content/uploads/formidable/13/2021-Casework-ProFlex-Thermal-Cycler-Validation-59019-1.pdf
Web link:

STRmix Validation with GlobalFiler and 3500 CE

Keywords: STRmix, Globalfiler, probabilistic genotyping
Laboratory: Colorado Bureau of Investigation
Discipline: Biology/Serology
Contact Name: Jennifer Malone, DNA Technical Leader
Email: jennifer.malone@nullstate.co.us
File link: https://www.ascld.org/wp-content/uploads/formidable/13/2018-STRmix-Validation_FINAL-38656-1.pdf
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A Quantum Leap in the Development of Quadratic External Calibration Models by EZSTATSG2, an Innovative Tool for ANSI/ASB Standard 036 Method Validation Using Microsoft Excel

Keywords: Method Validation, EZSTATSG2, ANSI/ASB Standard 036, ANOVA, Quadratic Calibration
Laboratory: Cuyahoga County Regional Forensic Science Laboratory at the Medical Examiner’s Office, Toxicology Department
Discipline: Toxicology
Contact Name: Szabolcs Sofalvi
Email: ssofalvi@nullcuyahogacounty.us
File link: https://www.ascld.org/wp-content/uploads/formidable/13/Preprint-AOV-EZSTATSG2-v20.pdf
Web link:

ArmedXpert Validation

Keywords: RMP, Random Match Probability
Laboratory: Ohio Bureau of Criminal Investigation
Discipline: Biology/Serology
Contact Name: Steven Wiechman
Email: Steven.Wiechman@nullohioattorneygeneral.gov
File link: https://www.ascld.org/wp-content/uploads/formidable/13/Ohio-BCI-ArmedXpert-Validation-12222020-signed.pdf
Web link:

Lumicyano Validation

Keywords: lumicyano, validation, fume, fuming, ardrox, super, glue, DNA, swabbing, methanol
Laboratory: San Bernardino County Sheriff’s Scientific Investigations Division
Discipline: Latent Prints
Contact Name: Kirk Garrison
Email: kgarrison@nullsbcsd.org
File link: https://www.ascld.org/wp-content/uploads/formidable/13/Scanned-Full-Study.pdf
Web link:

Implementing Hematoxylin into Casework at the North Carolina State Crime Laboratory

Keywords: hematoxylin, hair, root, stain, DNA
Laboratory: North Carolina State Crime Laboratory
Discipline: Trace Chemistry
Contact Name: Vanessa Martinucci
Email: vmartinucci@nullncdoj.gov
File link: https://www.ascld.org/wp-content/uploads/formidable/13/Hematoxylin-North-Carolina.pdf
Web link:

Yfiler Plus Validations

Keywords: Yfiler Plus, DNA,
Laboratory: MN Bureau of Criminal Apprehension Forensic Science Services
Discipline: Biology/Serology
Contact Name: Kris Deters
Email: kristine.deters@nullstate.mn.us
File link: https://www.ascld.org/wp-content/uploads/formidable/13/Biology-17-015-V.pdf
Web link:

Revised GRIM3 Validation

Keywords: GRIM, glass, refractive index, wavelength
Laboratory: MN Bureau of Criminal Apprehension Forensic Science Services
Discipline: Trace Chemistry
Contact Name: Patric Warrick
Email: patrick.warrick@nullstate.mn.us
File link: https://www.ascld.org/wp-content/uploads/formidable/13/Trace-15-001-V.pdf
Web link:

Confirmation of designer benzodiazepines in urine and blood on the 4500 QTrap LC-MS/MS system

Keywords: designer benzodiazepines, 4500 QTrap, NPS benzodiazepine
Laboratory: MN Bureau of Criminal Apprehension Forensic Science Services
Discipline: Toxicology
Contact Name: Brent Nelson
Email: brent.nelson@nullstate.mn.us
File link: https://www.ascld.org/wp-content/uploads/formidable/13/Toxicology-20-005-V.pdf
Web link:

Validation of the Thermo Fisher Gemini Handheld FT-IR & Raman Spectrometer

Keywords: Raman Spectrometer, controlled substances, packaging
Laboratory: MN Bureau of Criminal Apprehension Forensic Science Services
Discipline: Seized Drugs
Contact Name: Violet Stephens
Email: violet.stephens@nullstate.mn.us
File link: https://www.ascld.org/wp-content/uploads/formidable/13/Drug-Chemistry-18-002-V.pdf
Web link:

Validation of Microcrystal Tests for the Testing of Suspected Controlled Substances

Keywords: microcrystal, controlled substances, crystals,
Laboratory: MN Bureau of Criminal Apprehension Forensic Science Services
Discipline: Seized Drugs
Contact Name: Violet Stephens, Eric Grunwald
Email: violet.stephens@nullstate.mn.us
File link: https://www.ascld.org/wp-content/uploads/formidable/13/Drug-Chemistry-15-019-V.pdf
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Hair and Fiber Cross Contamination Prevention Study

Keywords: hair, fiber, tapelift, contamination, collection
Laboratory: Georgia Bureau of Investigation-Decatur
Discipline: Trace Chemistry
Contact Name: Tammy Jergovich
Email: tammy.jergovich@nullgbi.ga.gov
File link:
Web link:

Summary of Complex Mixture Evaluations

Keywords: DNA, Mixture, Complex
Laboratory: Maryland State Police Forensic Sciences Division
Discipline: Biology/Serology
Contact Name: Dan Katz
Email: daniel.katz@nullmaryland.gov
File link:
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Use of Mixture Deconvolution for the Purpose of Sole Source Match Statistics

Keywords: DNA, Mixture, Deconvolution
Laboratory: Maryland State Police Forensic Sciences Division
Discipline: Biology/Serology
Contact Name: Dan Katz
Email: daniel.katz@nullmaryland.gov
File link:
Web link:

Validation of the ISFG Mixture Analysis Recommendations

Keywords: DNA, Mixtures, ISFG
Laboratory: Maryland State Police Forensic Sciences Division
Discipline: Biology/Serology
Contact Name: Dan Katz
Email: daniel.katz@nullmaryland.gov
File link:
Web link:

ELISA Fentanyl Validation Report

Keywords: Fentanyl, ELISA
Laboratory: Palm Beach County Sheriff’s Office Crime Laboratory
Discipline: Toxicology
Contact Name: Tate Yeatman
Email: yeatmand@nullpbso.org
File link:
Web link:

Validation Report For Quantitation and/or Qualitative Identification of Benzodiazepines, Opioids, and Select Hypnotics by LC-MSMS

Keywords: LC-MSMS, Opioids, Benzodiazepines
Laboratory: Palm Beach County Sheriff’s Office
Discipline: Toxicology
Contact Name: Tate Yeatman
Email: yeatmand@nullpbso.org
File link:
Web link:

Seratec PSA Semiquant Test

Keywords: PSA, Seratec
Laboratory: Harris County Institute of Forensic Sciences
Discipline: Biology/Serology
Contact Name: Roger Kahn
Email: roger.kahn@nullifs.hctx.net
File link:
Web link:

AmpFlSTR® Identifiler® Plus PCR Amplification Kit

Keywords: Identifiler Plus, PCR, Amplification, Kit
Laboratory: Harris County Institute of Forensic Sciences
Discipline: Biology/Serology
Contact Name: Roger Kahn
Email: roger.kahn@nullifs.hctx.net
File link:
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