Validation & Evaluation Repository

The ASCLD Forensic Research Committee is proud to announce the launch of the Validation and Evaluation Repository. The goal is to compile a list of unique validations and evaluations conducted by forensic labs and universities. The repository will catalog these efforts and provide the contact information of the people responsible for the work. It is ASCLD’s hope that this listing will foster communication and reduce unnecessary repetition of validations and evaluations to benefit the forensic community. If you find a validation or evaluation that you would like more information about, simply use the contact information provided to request a copy.

In the May 2024 ANAB Heads Up Communication, they provided additional guidance about the ability to leverage another laboratory’s validation. “When another agency has performed a validation and shares the validation information other agencies can use the validation data to verify the method at their agency.”

 

Check out the complete article at: https://anab.ansi.org/resource/heads-up-for-forensic-accreditation/

 

If you would like to submit information for the Repository, please click HERE.

Please use the following items to search the entries. You can use as many of the following as you need:

Section

Section

Section

Section

The ASCLD Forensic Research Committee is proud to announce the launch of the Validation and Evaluation Repository. The goal is to compile a list of unique validations and evaluations conducted by forensic labs and universities. The repository will catalog these efforts and provide the contact information of the people responsible for the work. It is ASCLD’s hope that this listing will foster communication and reduce unnecessary repetition of validations and evaluations to benefit the forensic community. If you find a validation or evaluation that you would like more information about, simply use the contact information provided to request a copy.

In the May 2024 ANAB Heads Up Communication, they provided additional guidance about the ability to leverage another laboratory’s validation. “When another agency has performed a validation and shares the validation information other agencies can use the validation data to verify the method at their agency.”

 

Check out the complete article at: https://anab.ansi.org/resource/heads-up-for-forensic-accreditation/

 

If you would like to submit information for the Repository, please click HERE.

Please use the following items to search the entries. You can use as many of the following as you need:

Section

Section

Section

Section

Bullet Cleaning Solutions

Keywords: bullet, cleaning, bleach, Haemo-sol, OxiClean, ultrasonic
Laboratory: Kansas City Police Crime Laboratory
Discipline: Firearms/Toolmarks
Contact Name: Kyla Skinner
Email: kyla.skinner@kcpd.org
File link: https://www.ascld.org/wp-content/uploads/formidable/43/Bullet-Cleaning_Validation-Report.pdf
Web link:

YFiler Plus PCR Amplification Kit, cat no. 4484678, 4482730

Keywords: YFiler Plus, 3500
Laboratory: Mississippi Forensics Laboratory
Discipline: Biology/Serology
Contact Name: Mary Jones Dukes
Email: mjdukes@mcl.ms.gov
File link: https://www.ascld.org/wp-content/uploads/formidable/43/MSFL_YFP_VALIDATION-SUMMARY.122923.pdf
Web link:

Foster+Freeman RECOVER LFT Validation

Keywords: RECOVER LFT, cartridge cases, metal
Laboratory: Idaho State Police
Discipline: Latent Prints
Contact Name: Tina Walthall
Email: tina.walthall@isp.idaho.gov
File link:
Web link: https://isp.idaho.gov/forensics/validation/

Validation Report for Fire Debris Analysis by GC-MS

Keywords: Fire Debris, GCMS
Laboratory: Palm Beach County Sheriff’s Office Crime Laboratory
Discipline: Trace Chemistry
Contact Name: Tate Yeatman
Email: yeatmand@pbso.org
File link: https://www.ascld.org/wp-content/uploads/formidable/43/Validation-Report-for-Fire-Debris-Analysis-by-GC-MS.pdf
Web link:

Method Validation Report for the Identification and Quantitative Threshold Testing of Tetrahydrocannabinol (THC) in Cannabis

Keywords: THC, Cannabis, Hemp, Quantitative
Laboratory: Palm Beach County Sheriff’s Office Crime Laboratory
Discipline: Seized Drugs
Contact Name: Tate Yeatman
Email: yeatmand@pbso.org
File link: https://www.ascld.org/wp-content/uploads/formidable/43/Method-Validation-Report-for-the-Identification-and-Quantitative-Threshold-Testing-of-Tetrahydro.pdf
Web link:

Screening of Suspected Overdose Samples with Randox MultiSTAT Analyzer

Keywords: Toxicology, Immunoanalyser, Drugs of Abuse, Post-mortem Blood
Laboratory: Idaho State Police Forensic Services
Discipline: Toxicology
Contact Name: Tina Mattox
Email: tina.mattox@isp.idaho.gov
File link: https://www.ascld.org/wp-content/uploads/formidable/43/Randox-MultiSTAT-Validation-Plan-and-Summary-with-Attachments.pdf
Web link: http://www.isp.idaho.gov/

STRmix V2.6.2

Keywords: STRmix, Powerplex Fusion 6C, 3500
Laboratory: Palm Beach County Sheriff’s Office Crime Laboratory
Discipline: Biology/Serology
Contact Name: Amy McGuckian
Email: mcguckiana@pbso.org
File link: https://www.ascld.org/wp-content/uploads/formidable/43/Estimation-of-STRmix-V2.6-Parameters-for-Palm-Beach-County-Sheriffs-Office-PowerPlex-Fusion-6C-3.pdf, https://www.ascld.org/wp-content/uploads/formidable/43/PBSO-STRmix-v2.6.2-Internal-Validation-Powerplex-Fusion-6C-3500xl-CE.pdf, https://www.ascld.org/wp-content/uploads/formidable/43/STRmix-2.6.2-internal-functional-testing-evaluation-summary.pdf
Web link:

Internal Validation with PowerPlex Fusion 6C, Proflex PCR System, and 3500xL Series Genetic Analyzer Validation Report

Keywords: Fusion 6C, PowerPlex, ProFlex
Laboratory: Palm Beach County Sheriff’s Office Crime Laboratory
Discipline: Biology/Serology
Contact Name: Amy McGuckian
Email: mcguckiana@pbso.org
File link:
Web link: https://www.ascld.org/wp-content/uploads/formidable/13/Internal-Validation-with-PowerPlex-Fusion-6C-Proflex-PCR-System-and-3500xL-Series-Genetic-Analyz.pdf

Analysis of Benzodiazepines using Liquid-Chromatography Mass Spectrometry-Mass Spectrometry

Keywords: benzodiazepines, solid-phase extraction, LC/MS/MS
Laboratory: Wisconsin Division of Forensic Science
Discipline: Toxicology
Contact Name: Jonathan Tomko, Leah Macans or Toxicology Technical Lead
Email: tomkojt@doj.state.wi.us
File link: https://www.ascld.org/wp-content/uploads/formidable/43/Validation-Summary-for-Alcohol-Analysis-by-Headspace-GC-FIDMS.pdf, https://www.ascld.org/wp-content/uploads/formidable/43/Validation-Summary_20230310_final.pdf, https://www.ascld.org/wp-content/uploads/formidable/43/Supplementary-Document-One_20230302_final.pdf, https://www.ascld.org/wp-content/uploads/formidable/43/Validation-Pre-face_20230310_final.pdf, https://www.ascld.org/wp-content/uploads/formidable/43/TXPM-6.5-Benzodiazepines_Z-drugs-1097-9.pdf, https://www.ascld.org/wp-content/uploads/formidable/43/SOFT_2022_Abstract_Benzodiazpeines_220810.pdf
Web link: https://www.doj.state.wi.us/dfs/contact-information

Validation for the Qualitative Analysis of Seized Drugs by GC-IRD

Keywords: GC-IRD, Seized Drug, Cathinones
Laboratory: Palm Beach County Sheriff’s Office Crime Laboratory
Discipline: Seized Drugs
Contact Name: Ilene Alford
Email: alforda@pbso.org
File link:
Web link: https://www.ascld.org/wp-content/uploads/formidable/13/Validation-for-the-Qualitative-Analysis-of-Seized-Drugs-by-GC-IRD.pdf

Validation Report for Quantitation and/or Qualitative Identification of Benzodiazepines, Opioids and Select Hypnotics in Whole Blood by LC MSMS

Keywords: Opioids, Benzodiazepines, Hypnotics, LC-MSMS, Whole Blood
Laboratory: Palm Beach County Sheriff’s Office Crime Laboratory
Discipline: Toxicology
Contact Name: Nick Tiscione
Email: tiscionen@pbso.org
File link:
Web link: https://www.ascld.org/wp-content/uploads/formidable/13/Validation-Report-for-Quantitation-and_or-Qualitative-Identification-of-Benzodiazepines-Opioids-.pdf

Validation Report for Quantitation of Select Stimulants in Whole Blood by LC-MSMS

Keywords: Stimulants, LC-MSMS, Whole Blood
Laboratory: Palm Beach County Sheriff’s Office Crime Laboratory
Discipline: Toxicology
Contact Name: Nick Tiscione
Email: tiscionen@pbso.org
File link:
Web link: https://www.ascld.org/wp-content/uploads/formidable/13/Validation-Report-for-Quantitation-of-Select-Stimulants-in-Whole-Blood-by-LC-MSMS.pdf

Validation Summary for Alcohol Analysis by Headspace GC-FID/MS

Keywords: BAC, ethanol, Headspace
Laboratory: Palm Beach County Sheriff’s Office Crime Laboratory
Discipline: Toxicology
Contact Name: Nick Tiscione
Email: tiscionen@pbso.org
File link:
Web link: https://www.ascld.org/wp-content/uploads/formidable/13/Validation-Summary-for-Alcohol-Analysis-by-Headspace-GC-FIDMS.pdf

ForenSeq Kintelligence Kit, MiSeq FGx Sequencing, and Universal Analysis Software Internal Validation

Keywords: Kintelligence, Single nucleotide polymorphism (SNP), Massively Parallel Sequencing (MPS), Forensic genetic genealogy (FGG)
Laboratory: Signature Science, LLC – Center for Advanced Genomics
Discipline: Biology/Serology
Contact Name: Michelle Peck
Email: mpeck@signaturescience.com
File link:
Web link: https://www.biorxiv.org/content/10.1101/2022.10.28.514056v1%20

NYC OCME Internal Validation of STRmixTM v2.7 for Fusion 5C/3500xL Data – STRmixTM Parameters

Keywords: STRmix, Fusion 5C/3500XL
Laboratory: New York City Office of the Chief Medical Examiner
Discipline: Biology/Serology
Contact Name: Timothy D. Kupferschmid
Email: tkupferschmid@ocme.nyc.gov
File link:
Web link: https://www1.nyc.gov/assets/ocme/downloads/pdf/strmix_v2_7_parameters.pdf

Estimation of STRmix parameters for OCME New York Laboratory

Keywords: STRmix
Laboratory: New York City Office of the Chief Medical Examiner
Discipline: Biology/Serology
Contact Name: Timothy D. Kupferschmid
Email: tkupferschmid@ocme.nyc.gov
File link:
Web link: https://www1.nyc.gov/assets/ocme/downloads/pdf/strmix_parameters.pdf

Internal Validation of STRmix V2.4 for Fusion

Keywords: STRmix
Laboratory: New York City Office of the Chief Medical Examiner
Discipline: Biology/Serology
Contact Name: Timothy D. Kupferschmid
Email: tkupferschmid@ocme.nyc.gov
File link:
Web link: https://www1.nyc.gov/assets/ocme/downloads/pdf/STRmix-V2-4-Fusion-5C-Validation%20Summary.pdf

Internal Validation of GeneMarker HID software

Keywords: GeneMarker, HID
Laboratory: New York City Office of the Chief Medical Examiner
Discipline: Biology/Serology
Contact Name: Timothy D. Kupferschmid
Email: tkupferschmid@ocme.nyc.gov
File link:
Web link: https://www1.nyc.gov/assets/ocme/downloads/pdf/GeneMarker-HID-Validation-Summary.pdf

PowerPlex® Fusion System Amplification Kit on the Applied Biosystems 3130xl Genetic Analyzer using GeneMarker HID v. 2.8.2 Analysis Software Validation Report

Keywords: PowerPlex Fusion, 3130xL
Laboratory: New York City Office of the Chief Medical Examiner
Discipline: Biology/Serology
Contact Name: Timothy D. Kupferschmid
Email: tkupferschmid@ocme.nyc.gov
File link:
Web link: https://www1.nyc.gov/assets/ocme/downloads/pdf/Fusion-Validation-Summary.pdf

Internal Validation of STRmix™ v2.7 for Fusion 5C/3500xL Data

Keywords: STRmix, Fusion 5C/3500xL
Laboratory: New York City Office of the Chief Medical Examiner
Discipline: Biology/Serology
Contact Name: Timothy D. Kupferschmid
Email: tkupferschmid@ocme.nyc.gov
File link:
Web link: https://www1.nyc.gov/assets/ocme/downloads/pdf/internal_validation_strmix_2_7.pdf

Multi-drug Screen in Blood and Urine by LC/MS/MS Using SLE+ Extraction Plates

Keywords: Toxicology, Multi-Drug, LC/MS/MS
Laboratory: Idaho State Police Forensic Services
Discipline: Toxicology
Contact Name: Matthew Gamette
Email: matthew.gamette@isp.idaho.gov
File link: https://www.ascld.org/wp-content/uploads/formidable/43/Validation-Summary-MDS-APPROVED-Rev.pdf
Web link: https://isp.idaho.gov/forensics/validation/

Multi-drug and Cannabinoids Screen in Blood and Urine by LC/Q-TOF Using SLE+ Extraction Plates

Keywords: Toxicology, LC/Q-TOF, Multi-Drug, Cannabinoids
Laboratory: Idaho State Police Forensic Services
Discipline: Toxicology
Contact Name: Matthew Gamette
Email: matthew.gamette@isp.idaho.gov
File link: https://www.ascld.org/wp-content/uploads/formidable/43/Final-Validation-Summary-QTOF-MDS-APPROVED-Rev.pdf, https://www.ascld.org/wp-content/uploads/formidable/43/Final-Validation-Summary-QTOF-Cannabinoids-APPROVED-Rev.pdf
Web link: https://isp.idaho.gov/forensics/validation/

Antiepileptics / Acidic Drug Confirmation and Quantitation by LCMSMS

Keywords: antiepileptics, acidic drugs, LCMSMS
Laboratory: Montgomery County Coroner’s Office / Miami Valley Regional Crime Laboratory
Discipline: Toxicology
Contact Name: Matthew Juhascik
Email: juhascikm@mcohio.org
File link: https://www.ascld.org/wp-content/uploads/formidable/43/AEA-by-LCMSMS-Summary-of-Validation-7482-1-1-Rev.pdf
Web link:

Hemp analysis by LCMSMS

Keywords: hemp, marijuana, total THC, LCMSMS
Laboratory: Montgomery County Coroner’s Office / Miami Valley Regional Crime Laboratory
Discipline: Seized Drugs
Contact Name: Matthew Juhascik
Email: juhascikm@mcohio.org
File link: https://www.ascld.org/wp-content/uploads/formidable/43/Hemp-THC-and-THCA-by-LCMSMS-Summary-of-Validation-7489-1-Rev.pdf
Web link:

Antiepileptics / Acidic Drug Confirmation and Quantitation by LCMSMS

Keywords: antiepileptics, acidic drugs, LCMSMS
Laboratory: Montgomery County Coroner’s Office / Miami Valley Regional Crime Laboratory
Discipline: Toxicology
Contact Name: Matthew Juhascik
Email: juhascikm@mcohio.org
File link: https://www.ascld.org/wp-content/uploads/formidable/43/AEA-by-LCMSMS-Summary-of-Validation-7482-1-Rev.pdf
Web link:

Drug-Facilitated Crime Analysis by LCMSMS

Keywords: drug-facilitated, sexual assault, LCMSMS, solid-phase extraction
Laboratory: Montgomery County Coroner’s Office / Miami Valley Regional Crime Laboratory
Discipline: Toxicology
Contact Name: Matthew Juhascik
Email: juhascikm@mcohio.org
File link: https://www.ascld.org/wp-content/uploads/formidable/43/DFC-Validation-Summary-7487-1-Rev.pdf
Web link:

THC related compounds by LCMSMS

Keywords: THC, delta 8, LCMSMS, solid-phase extraction
Laboratory: Montgomery County Coroner’s Office / Miami Valley Regional Crime Laboratory
Discipline: Toxicology
Contact Name: Matthew Juhascik
Email: juhascikm@mcohio.org
File link: https://www.ascld.org/wp-content/uploads/formidable/43/THCPlus-by-LCMSMS-Summary-of-Validation-7494-1-Rev.pdf
Web link:

Benzodiazepines by LCMSMS

Keywords: Benzodiazepines, LCMSMS, solid-phase extraction
Laboratory: Montgomery County Coroner’s Office / Miami Valley Regional Crime Laboratory
Discipline: Toxicology
Contact Name: Matthew Juhascik
Email: juhascikm@mcohio.org
File link: https://www.ascld.org/wp-content/uploads/formidable/43/Benzo-by-LCMSMS-Summary-of-Validation-7486-1-Rev.pdf
Web link:

ProFlex Thermalcycler Validation with GlobalFiler

Keywords: ProFlex, PCR, Thermalcycler, Globalfiler, Yfiler
Laboratory: Colorado Bureau of Investigation
Discipline: Biology/Serology
Contact Name: Jennifer Malone, DNA Technical Leader
Email: jennifer.malone@state.co.us
File link: https://www.ascld.org/wp-content/uploads/formidable/43/2021-Casework-ProFlex-Thermal-Cycler-Validation-59019-1-Rev.pdf
Web link:

STRmix Validation with GlobalFiler and 3500 CE

Keywords: STRmix, Globalfiler, probabilistic genotyping
Laboratory: Colorado Bureau of Investigation
Discipline: Biology/Serology
Contact Name: Jennifer Malone, DNA Technical Leader
Email: jennifer.malone@state.co.us
File link: https://www.ascld.org/wp-content/uploads/formidable/43/2018-STRmix-Validation_FINAL-38656-1-Rev.pdf
Web link:

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Outstanding Evaluation/Validation Award Winner!

The award will recognize an outstanding evaluation/validation study that has been submitted to the FRC Evaluation/Validation Repository within the last two years.

Check Out Our

Outstanding Evaluation/Validation Award Winner!

The award will recognize an outstanding evaluation/validation study that has been submitted to the FRC Evaluation/Validation Repository within the last two years.